Biotin is an important vitamin functioning as coenzyme in several carboxylase-mediated metabolic reactions. Total 105 foods were selected as the target samples, of those 75 samples were highly and frequently consumed by Korean and the others were potently high biotin content foods. HPLC with the triple column system had been optimized and applied for the analysis of biotin in various food samples. The foods with the biotin concentration over $0.10{mu}g/g$ were 13 kinds of foods such as soybean ($2.037{mu}g/g$), soy milk ($0.591{mu}g/g$), pork liver ($0.455{mu}g/g$), etc. The total daily biotin exposure per person via average and the $95^{th}$ percentile food intake was 44.656 and $169.416{mu}g$, respectively. The Korean staple food, rice contributed as fifth diet for biotin intake ($3.433{mu}g/person/day$) following soybean ($9.167{mu}g/person/day$) > milk ($6.018{mu}g/person/day$)> soy milk ($4.433{mu}g/person/day$) > beer ($4.428{mu}g/person/day$).