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Detection of Feline Calicivirus as Norovirus Surrogate in Food and Water Sources Using Filtration and Real-time RT-PCR
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  • Detection of Feline Calicivirus as Norovirus Surrogate in Food and Water Sources Using Filtration and Real-time RT-PCR
  • Detection of Feline Calicivirus as Norovirus Surrogate in Food and Water Sources Using Filtration and Real-time RT-PCR
저자명
Cho. Min-Gyu,Jeong. Hye-Mi,Ahn. Jun-Bae,Kim. Kwang-Yup
간행물명
Food science and biotechnology
권/호정보
2011년|20권 6호|pp.1475-1480 (6 pages)
발행정보
한국식품과학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Norovirus (NoV) is a major cause of acute non-bacterial gastroenteritis in all age groups worldwide. To detect NoV from foods, polyethylene glycol (PEG) precipitation or ultracentrifugation methods are generally used with reverse transcription (RT)-PCR. These methods need to use complicated procedures and varied buffers depending on the kinds of food matrices. In this study, we suggested a universal method to recover NoV in food and water samples as a prior step to real-time RT-PCR. As a NoV surrogate model, feline calicivirus (FCV) was used. FCV was artificially inoculated to samples, and then concentrated by the adsorption-elution method using negatively charged membrane filters. The detection limit was $4.3{ imes}10^1$ PFU/250 mL for distilled water, $4.3{ imes}10^2$ PFU/250 mL for environmental waters, and $4.3{ imes}10^2$ PFU/ 15 g for lettuce and oyster. We were able to identify the possibility of one universal and time-saving method to detect NoV in food and water samples without modifications.