To provide the operation rule curve for suppling instream flow to urban stream from reservoir, the Soho reservoir with watershed area of 7.4 $km^2$ and total water storage of 2.58 $Mm^3$ was planned at the headwaters of the Daejeoncheon. Daily streamflow was simulated and using the simulated streamflow and desired instream flow, the operation rule curve by Senga method was drawn and evaluated through reservoir operation. Senga method is derived by accumulating the differences between streamflow and desired instream flow adversely. Water storages were simulated on a daily basis to supply urban instream flow from Soho reservoir, but the amount of supplying instream flow to urban stream was not nearly increased comparing with that of normal operation that does not used the rule curve. Thereafter the new simulation-based operation rule curve was derived and applied to supply instream flow from Soho reservoir. In normal operation, the amount of instream flow was shown to 15,000 $m^3$/d, but it was increased to 27,700 $m^3$/d in withdrawal limited operation using the new derived rule curve, in which the applicability of this rule curve was proved. Also comparing with the flow duration curves at station just before urban Daejeoncheon stream without and with upstream Soho reservoir, the 95th flow was decreased from 1.64 mm/d to 1.51 mm/d, and the 355th flow was increased from 0.17 mm/d to 0.30 mm/d. Monthly streamflows during October to March were increased from 10.6~24.1 mm to 24.1~34.0 mm with the increasing rate of 141~227%.