- 황련해독탕 중 3종 생리활성 물질의 HPLC-DAD 동시 정량분석법 확립
- ㆍ 저자명
- 양혜진,원진배,마진열,마충제,Yang. Hye-Jin,Weon. Jin-Bae,Ma. Jin-Yeul,Ma. Choong-Je
- ㆍ 간행물명
- 약학회지
- ㆍ 권/호정보
- 2011년|55권 1호|pp.64-68 (5 pages)
- ㆍ 발행정보
- 대한약학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
In this study, a high performance liquid chromatography-diode array detector method was established, for simultaneous determination of three compounds, berberine, palmatine and geniposide in Hwangryunhaedok-tang, To develop and validate method, $C_{18}$ column (5 ${mu}M$, 4.6 mm${ imes}$250 mm) was used with gradient mobile phase, water containing 0.1% trifluoroacetic acid (TFA) and MeOH at the column temperature of $30^{circ}C$. UV wavelength was set at 230 and 280 nm. Validation of the chromatography method was evaluated by linearity, precision and accuracy test. Calibration curve of standard components showed good linearity ($R^2$ > 0.9999). The limits of detection (LOD) and limits of quantification (LOQ) varied from 0.05 to 0.17 ${mu}g/ml$ and 0.15 to 0.53 ${mu}g/ml$, respectively. The relative standard deviations (RSDs) data of intra-day and inter-day test were in less than 2.99% and 1.90%, respectively. The results of the accuracy test were in the range of 98.36 to 102.52% with RSDs values 0.32 to 1.98%. The results of validation indicated that this method was a very accurate and sensitive assay.