An experiment was conducted to characterize the effects of SOF on EGR cooler fouling. A removable single-tube test rig combined with a soot generator was developed to represent an EGR cooler and diesel exhaust gas. The use of a soot generator, which controlled the size and concentration of soot particles, enabled independent variables to be completely controlled. Either n-dodecane or diesel lube oil as substitute SOFs were vaporized and injected into the test rig to evaluate their effects on the growth of PM deposits and the degradation performance of the EGR cooler. Coolant temperature, which seemed to be associated with SOF content, was chosen as an independent variable, and PM deposit mass per unit area and the effectiveness drop versus time increased as the coolant temperature decreased. The PM deposit mass per unit area and effectiveness drop had maximum values at a coolant temperature of 40oC for every n-dodecane injection rate. For substitute SOFs tested in this experiment, the deposit mass increased when either n-dodecane or diesel lube oil was injected, but the effect of lube oil was more significant. Diesel lube oil seemed to have a stronger effect on the reduction of thermal conductivity by filling pores in the deposits. When diesel lube oil was injected, the deposit mass per unit area increased 127% compared to dry soot without injection. The effectiveness drop after 10 hours increased only 12.5%.