A novel particle removal system for air handling unit (AHU) of subway station was evaluated experimentally. The novel system was designed in order to minimize the maintenance cost by applying axial-flow cyclones. The system consists of multiple cyclone units and dust trap. Based on our previous numerical study, it was found to be effective for removal $1sim10{mu}m$ sized dust particles. In this study, we manufactured the mock-up model and evaluated the model experimentally. Liquid and solid test particles were generated for evaluating collection efficiency of the system and the pressure drop was monitored. The collection efficiency was varied from 41.2% to 85.9% with increasing the sizes of particle from 1 to $6.5{mu}m$ by particle count ratio of inlet and outlet. The pressure drop was maintained constant less than $20mmH_2O$. In addition, the collection efficiency was estimated by total mass for solid test particles. It was found that the collection efficiency was 65.7% by particle mass ratio of inlet and outlet. It shows that present system can replace current pre-filters used in subway HVAC system for removing particulate matters with minimal operational cost.