Objectives : The aim of this study was to examine the effect of manual acupuncture at the bilateral $LI_4$, $ST_{36}$ and $LR_3$ on normal human every 3 minute time interval by using power spectrum analysis. Methods : 25 healthy volunteers participated in this study. 32 channel EEG(Electroencephalogram) measurements were carried out before, during and after acupuncture treatments. In this study, power spectrum was used as a measure of complexity. Statistical analysis was performed using repeated measure ANOVA and Bonferroni method. Results : 1. In acupuncture group, Mean HRT, PSI, Normalized HF decreased significantly. SDNN, Ln(TP), Ln(LF), Ln(VLF), Normalized LF and LF/HF ratio increased significantly. RMSSD, Ln(HF) were not affected. 2. In sham acupuncture group, all indicators were not affected. 3. In control group, mean HRT, Ln(TP) decreased significantly. SDNN, RMSSD, PSI, Ln(LF), Ln(HF), Ln(VLF), normalized LF, normalized HF and LF/HF ratio were not affected. Conclusions : This results suggest that manipulation needling of 6 acupoints induced increase of delta, theta, beta waves. Both manipulation and simple needling induced decrease of alpha wave during 12~15 time intervals.