In this paper, we propose a hand image segmentation method using the dynamic threshold values on input images with various lighting and background attributes. First, a moving hand silhouette is extracted using the camera input difference images, Next, based on the R,G,B histogram analysis of the extracted hand silhouette area, the threshold interval for each R, G, and B is calculated on run-time. Finally, the hand area is segmented using the thresholding and then a morphology operation, a connected component analysis and a flood-fill operation are performed for the noise removal. Experimental results on various input images showed that our hand segmentation method provides high level of accuracy and relatively fast stable results without the need of the fixed threshold values. Proposed methods can be used in the user interface of mixed reality applications.