The purpose of this study was to compare metabolic energy expenditure with the computed kinetic energy for different speeds of walking and running over the treadmill and to find the relevance for individual and group equation by performing a statistical analysis, Bland-Altman plot. Seven male subjects participated, and they were required to walk and run on the treadmill with the gas analyzer and triaxial accelerometer. Walking speeds were 3.0, 4.0, 5.0 and 6.0 km/h and running speeds were 7.0, 8.0 and 9.0 km/h respectively. Kinetic energy was calculated by the integration of acceleration data and compared with the metabolic energy measured by a gas analyzer. Correlation coefficients showed relatively good between the measured metabolic energy and the calculated kinetic energy. In addition, a dramatic increase in kinetic energy was also observed at the transition speed of walking and running, and two standard deviations in Bland-Altman plot, derived from the difference between measured and predicted values, were 1.14, 2.53, 2.93, 1.80, 2.80, 0.60 and 2.48 respectively. It was showed that there is no difference for methods of how to predict the kinetic energy expenditure for individual and group even though people had each different physical characteristic.