Inhibitory effects of lipid accumulation by citrus peel extract were evaluated. Citrus peel extract (CPE) ($500{mu}g/mL$) inhibited lipid and triglyceride accumulation in 3T3-L1 adipocytes by more than 64.5 and 58.7%, respectively and suppressed glycerol-3-phosphate dehydrogenase to the levels of preadipocytes. The expression of perilipin mRNA in 3T3-L1 adipocytes treated with CPE was significantly lower than that in untreated adipocytes (p<0.05), with no accompanying increase in lipolysis-related mRNA. Flavonoid compositions of CPE were hesperidin 13.79, narirutin 7, and naringin $262.5{mu}g/g$, respectively. These findings suggest CPE may indirectly stimulate lipolysis by inhibiting protection of physical barrier on lipid droplet to hinder lipid accumulation in 3T3-L1 adipocytes.