An isolate of actinomycetes was obtained from farmland soil and designated as Streptomyces sp. A1022 based on cultural characteristics, 16S rDNA, DNA gyrase subunit B (gyrB), and fatty acid analyses. When strain A1022 was grown on chrome azurol S agar plate, it did not produce siderophore. However, strain A1022 had a strong antagonism and broad spectrum against six out of nine tested phytopathogens including Alternaria logipes, Cercospora canescece, Colletotrichum gloeosporioides, Diaporthe citri, Magnaporthe grisea, and Sclerotinia soleroforum. These results suggest that Streptomyces sp. A1022 could be employed as a potential biocontrol agent for the development of biopesticide having broad spectrum.