This study was to examine the positive and negative affect related to psychological characteristics of each Sasang types using Positive and Negative Affect Schedule (PANAS) with 78 students from School of Korean Medicine, Pusan National University. After determining the Sasang types by QSCC II, the psychological characteristics of each Sasang types were analysed by PANAS. We did ANOVA analysis with eight related scales and found significant differences between the Tae-Eum type ($18.71{pm}8.75$, $6.47{pm}3.36$, $6.31{pm}2.47$, $3.18{pm}2.77$, $10.94{pm}10.08$) and the So-Eum type ($13.38{pm}6.03$, $4.55{pm}2.23$, $4.19{pm}2.16$, $5.81{pm}4.20$, $1.83{pm}9.17$, respectively) in Positive Affect (PA) (F=3.931, p=0.024), PA-Joy (F=3.991, p=0.023), PA-Interest (F=5.025, p=0.009), NA-Afraid (F=3.118, p=0.050), and Difference between Positive and Negative Affect (DPN) (F=6.355, p=0.003) scores. The post-hoc analysis showed that the Tae-Eum type ($18.71{pm}8.75$, $6.47{pm}3.36$, $6.31{pm}2.47$, $10.94{pm}10.08$) has significantly higher score than So-Eum type ($13.38{pm}6.03$, $4.55{pm}2.23$, $4.19{pm}2.16$, $1.83{pm}9.17$, respectively) in PA (p=0.018), PA-Joy (p=0.021), PA-Interest (p=0.017), and DPN (p=0.002) scores. The So-Eum type ($5.81{pm}4.20$) showed significantly (p=0.047) higher score than the Tae-Eum type ($3.18{pm}2.77$) in NA-Afraid. Results demonstrated distinct affect profile differences between Tae-Eum and So-Eum Sasang types using PANAS. This study may serve as the foundation in identifying psychological traits of Sasang types, especially regarding the aspect of affect.