Purpose: The purpose of this study was to investigate onset timing of vastus medialis oblique(VMO) relative to vastus lateralis(VL) the VMO/VL electromyographic(EMG) ratio according to heel height Methods: A repeated measures design was used. Fifteen healthy female college students with no known knee musculoskeletal dysfunction were recruited this study. They carried out a standardised stair acent activity under 4 conditions; barefoot, and with heel height of 1, 3, 7 cm. Muscle activity was measured by surface EMG (Myosystem 1400A, Noraxon Inc., U.S.A). Data were analysed using $1{ imes}4$ repeated measures ANOVA. Results: Onset timing differed with heel height(p<.05). However, the VMO/VL EMG ratio was not significantly difference between conditions. Conclusion: We found that 7 cm heel height delayed in VMO onset compared with 3 cm heel height during stair ascent, but no change in the relative EMG intensity of VMO and VL as measured by th VMO/VL ratio. The findings indicate that high-heeled shoes may have disadvantages in knee stability because of delayed onset of VMO. Due to a lack of knee joint stability, wearing of high heeled shoes should be avoided.