Daphnia Magna Stratus has been widely accepted as useful species for estimating the toxicity of chemicals to aquatic invertebrate and recommended as species for the testing chemicals from the international guideline as well as Korean guideline. The study was performed for the acute toxicity test by using water flea(D. Magna) for effluents from several wastewater treatment plants and drainage canals in GyeongBuk area. Five heavy metals, 1,4-Dioxane and Perchlorate were tested. Most Toxicity Units(TU) of Industrial wastewater effluents were less than 1 which means effluent was not toxic to D. Magna. However, effluents containing 1,4-Dioxane and Perchlorate were significantly toxic to D. Magna. Therefore, facilities should reduce the 1,4-dioxane since new regulations will force them after the year of 2011.