A pose estimation process from medical images is calculating locations and orientations of objects obtained from Computed Tomography (CT) volume data utilizing X-ray images from two directions. In this process, digitally reconstructed radiograph (DRR) images of spatially transformed objects are generated and compared to X-ray images repeatedly until reasonable transformation matrices of the objects are found. The DRR generation and image comparison take majority of the total time for this pose estimation. In this paper, a fast DRR generation technique based on GPU parallel computing is introduced. A volume ray-casting algorithm is explained with brief vector operations and a parallelization technique of the algorithm using Compute Unified Device Architecture (CUDA) is discussed. This paper also presents the implementation results and time measurements comparing to those from pure-CPU implementation and open source toolkit.