Objective: The purpose of this study was to analyze the risk factors of the musculoskeletal workload of mid-old-age female caregivers by identifying work postures and subjective symptoms. Method: This study was carried out on a total of 206 subjects 206 mid-old-age caregivers(45~65 years old) from November 2009 to April 2010: 206 mid-old-age caregivers (45~65 years old) at the long-term hospital. A questionnaires survey on general factors and subjective symptoms of subjects was conducted. The risk factors of the musculoskeletal workload evaluation method is based on OWAS, RULA, REBA according to occupation properties. Results: The analysis of the rates of the subjective symptoms showed that 78.6% subjects experience low back pain. The analysis of the work type showed that transfer is the highest rate(36.3%) and position change is the second(18.2%). The analysis of the work postures showed that the thrusted back with twisting position over $20^{circ}$ is highest rate(37.4%), the bended back forward with twisting position is the second(27.5%). Conclusion: This study suggested that the need of preventive education for caregivers and workload improve. It is hoped that subsequent study on the difference of subjective symptoms between educated caregivers and non educated caregivers will be conducted.