The purpose of this study was to examine the relationships between temperament, social support and positive, as well as negative emotions of institutionalized children. The participants were 198 children aged from 10 to 13 years old. They were recruited from 20 childcare facilities in Seoul. Data were analyzed using Cronbach alphas, factor analyses, frequencies, percentiles, t-tests and hierarchical regression analyses. The major findings were as follows: 1) on average, girls showed higher emotionality in temperament compared to boys. Girls reported greater support of school teacher than boys. Boys experienced significantly higher positive emotions than girls, while girls reported significantly higher negative emotions than boys. 2) Positive emotions were influenced by the support of the institutionalized family as well as school teachers. Negative emotions were influenced by the support of the institutionalized family.