This study intends to assess the defect in the weld zone of titanium grade 2 plate in terms of acoustical anisotropy based on the angle beam method. Depending on the rolling direction, the ratio of wave velocity was found to be 1.08 and the difference in the angle of refraction was more than seven degrees, confirming the presence of acoustical anisotropy. Thus for measuring the length of defect in the weld zone of the titanium plate (thickness of 10mm), the distance amplitude characteristics curves of titanium, TDACC-R and TDACC-T were constructed for the measurements in consideration of the acoustical anisotropy on CRT of the ultrasonic testing equipment. As a result, when the distance amplitude characteristics curve corresponds to the rolling direction, the length of defect was close to the actual measurement within 1mm and when different, the difference was found to be over 4mm. It was affirmed that the acoustical anisotropy should be taken into consideration when measuring the length of defects in the weld zone of the titanium plate with the presence of acoustical anisotropy.