Ginsenosides, one of the well-known traditional herbal substance, frequently are used in South Korea for the prevention of cardiovascular disease. The effects of ginsenoside-Re (G-Re) on ischemia-induced heart were investigated through analyses of hemodynamic change including perfusion pressure, aortic flow, coronary flow, and cardiac output. Myocardial function was recorded. Administration of ginsenoside-Re significantly prevented decreases in perfusion pressure, aortic flow, coronary flow, and cardiac output. The Administration of G-Re significantly recovered all of the hemodynamic parameters, except heart rate, after ischemia-reperfusion (I/R) injury (perfusion pressure: $78.49{pm}3.98%$ vs. $66.61{pm}3.3%$, aortic flow volume: $59.26{pm}2.29%$ vs. $42.68{pm}2.17%$, coronary flow volume: $74.89{pm}2.88%$ vs. $52.89{pm}2.81%$, and cardiac output: $63.12{pm}3.81%$ vs. $45.25{pm}3.83%$, P<0.01, respectively, normal control as 100%). The intracellular calcium ($[Ca^{2+}]_i$) content in cardiomyocytes was quantitatively determined. Administration of G-Re significantly prevented $[Ca^{2+}]_i$ increase that had been induced by simulated I/R in vitro (P<0.01) in a dose-dependent manner, suggesting that the antiischemic role of G-Re is mediated by the inhibition of $[Ca^{2+}]_i$ increase. Over all, we found that the administration of G-Re has cardioprotective effects on the isolated heart. The beneficial effects may be mediated through controlling the level of $[Ca^{2+}]_i$. These results indicate that G-Re has a distinct cardioprotective effect in an isolated rat heart.