Objectives : Zingberis Rhizoma (ZR) and Glycyrrihizae Radix (GR) have been widely used to prevent or treat allergic diseases. However, relatively little research has been conducted on the immune response to Senggang Gamcho-tang (SG) in a mixture of ZR and GR. The purpose of this study was to determine antiallergic effects of Senggang Gamcho-tang (SG) extracts ZR and GR on activity of murine splenocytes. Methods : This study was performed to investigate the effect of SG in mice, using in vitro experiments. Cells were treated with SG extract (1 ${mu}g$/ml, 10 ${mu}g$/ml and 100 ${mu}g$/ml) plus Con A (2 ${mu}g$/ml) and ZR (6.6 ${mu}g$/ml and 66 ${mu}g$/ml), GR (3.3 ${mu}g$/ml and 33 ${mu}g$/ml) respectively for 48 hrs. The production of IFN-${gamma}$ and IL-4 were determined by ELISA. Results : No toxicity was found in splenocytes treated with SG extract for 48 hrs at the concentration of 0 ${mu}g$/ml, 1 ${mu}g$/ml, 10 ${mu}g$/ml and 100 ${mu}g$/ml, respectively. The production of IFN-${gamma}$ increased to 23,968.7 pg/ml (p<0.001) while that of IL-4 significantly decreased to 14.1 pg/m l(p<0.05) only at the SG concentration of 100 ${mu}g$/ml. With the GR concentration of 33 ${mu}g$/ml in SG extract, the production of IFN-${gamma}$ rose to 32,102.2 pg/ml (p<0.001) while that of IL-4 (p<0.05) declined to 34.2 pg/ml. However, there was no significant observation with regard to the effect of ZR on production of IFN-${gamma}$ and IL-4. Conclusions : This study suggests an antiallergic effect of Senggang Gamcho-tang, which leads to the implication that SG extract may be used to enhance human immune function.