Objective: The objective of this study was to compare one-hand and two-hands lifting activity in terms of biomechanical stress for the range of lifting heights from 10cm above floor level to knuckle height. Background: Even though two-hands lifting activity of manual materials handling tasks are prevalent at the industrial site, many manual materials handling tasks which require the worker to perform one-hand lifting are also very common at the industrial site and forestry and farming. Method: Eight male subjects were asked to perform lifting tasks using both a one-handed as well as a two-handed lifting technique. Trunk muscle electromyographic activity was recorded while the subjects performed the lifting tasks. This information was used as input to an EMG-assisted free-dynamic biomechanical model that predicted spinal loading in three dimensions. Results: It was shown that for the left-hand lifting tasks, the values of moment, lateral shear force, A-P shear force, and compressive force were increased by the average 43%, as the workload was increased twice from 7.5kg to 15.0kg. For the right-hand lifting task, these were increased by the average 34%. For the two-hands lifting tasks, these were increased by the average 25%. The lateral shear forces at L5/S1 of one-hand lifting tasks, notwithstanding the half of the workload of two-hands lifting tasks, were very high in the 300~317% of the one of two-hands lifting tasks. The moments at L5/S1 of one-hand lifting tasks were 126~166% of the one of two-hands lifting tasks. Conclusion: It is concluded that the effect of workload for one-hand lifting is greater than two-hands lifting. It can also be concluded that asymmetrical effect of one-hand lifting is much greater than workload effect. Application: The results of this study can be used to provide guidelines of recommended safe weights for tasks involved in one-hand lifting activity.