Muscle fatigue is characterized as a progressive increase in discomfort arising from the active muscle at moderate load levels are maintained. The median frequency is the most commonly used as a parameter to describe muscle fatigue. However, the estimate of the median frequency is difficult to indicate muscle fatigue because of its high standard deviation and instability. This paper investigates the power changes of the appropriate low frequency band as a fatigue parameter in EMG during isotonic exercise. To select the appropriate band, linear regression lines are employed to calculate the slopes and the coefficient of determination. Three females and seven males volunteered to participate in surface EMG recordings placed on the biceps brachii and each recording experiment continued until their exhaustion. The results of experiment shows that the power changes of the selected low frequency band (15~45 Hz) have linear slopes and high determinant coefficients. Therefore, this fatiguing parameter using the power changes of the low frequency band is valid to measure the state of muscular fatigue.