Recently, various in-vehicle networks have been developed respectively in order to accomplish their own purposes such as CAN and MOST. Especially, the MOST network is usually adapted to provide entertainment service. The car navigation system is also widely used for guiding driving paths to driver. The position for the navigation system is usually acquired by GPS technology. However, the GPS technique has two serious problems. The first is unavailability in urban canyons. The second is inherent positional error rate. The problems have been studied in many literatures. However, the second still leads to incorrect locational information in some area, especially parallel roads. This paper proposes a performance tuning method of image matching algorithm for the car navigation system. The method utilizes images obtained from in-vehicle MOST network and a real-time image matching algorithm which determines the direction of moving vehicle in parallel section of road. In order to accuracy improvement of image matching algorithm, three conditions are applied. The experimental tests show that the proposed system increases the accuracy.