In this paper, we propose an adaptive thresholding method to binarize two-dimensional barcode images. Adaptive thresholding methods that minimize light effects convert an original image into a binary image. The methods are applied to document image binarization. The methods, however, have problems of determining box size used in adaptive thresholding. thus, they inappropriate to use in recognition of two-dimensional barcode images. To overcome the problem, we analysis the problem and propose a new adaptive threshold method using the integral image. To show the effectiveness of our method, we compared our method with the well-known existing methods in terms of visual quality and processing time. The experimental result indicates that the proposed method is superior to the existing method.