Applicability of 8 day interval flow data for the calibration of hydrologic model was evaluated using Hydrological Simulation Program-Fortran (HSPF) at Kyungan watershed. The 8 day interval flow monitored by Ministry of Environment located at upstream was calibrated and periodically validated during 2004-2008. And continuous daily flow monitored by Ministry of Construction & Transportation (MOCT) and located at the mouth was compared with daily simulated data during 2004-2007 as spatial validation. Automatic calibration tool which is Model-Independent Parameter Estimation & Uncertainty Analysis (PEST) was applied for HSPF calibration procedure. The model efficiencies for calibration and periodic validation were 0.63 and 0.88, and model performances were fair and very good, respectively, based on criteria of calibration tolerances. Continuous daily stream flow at the mouth of Kyungan watershed were good agreement with observed continuous daily stream flow with showing 0.63 NS value. The PEST program is very useful tool for HSPF hydrologic calibration using non-continuous daily stream flow as well as continuous daily stream flow. The 8 day interval flow data monitored by MOE could be used to calibrate hydrologic model if the continuous daily stream flow is unavailable.