Differential absorption LIDAR (DIAL) is frequently used for atmospheric gas monitoring to detect impurities such as nitrogen dioxide, sulfur dioxide, iodine, and ozone. However, large differences in the on- and off-line laser wavelengths can cause serious errors owing to differential aerosol scattering. To resolve this problem, we have developed a new DIAL system for iodine vapor measurements in particular. The suggested DIAL system uses only one laser under seeded and unseeded conditions. To check the detection-sensitivity and error effects, we compared the results from a system using two seeded lasers with those from a system using a seeded and an unseeded laser. We demonstrate that the iodine concentration sensitivity of our system is improved in comparison to the conventional two seeded or two unseeded laser combinations.