Purpose: The purpose of this study was to examine the effects of line dance program on muscle strength, balance, flexibility, pain, and mood state in post-menopausal women. Methods: A quasi-experimental research design (one group pretest-posttest) was employed. A total of 13 post-menopausal women completed 12-week line dance program(60min/day, 2days/wk) in S city. Descriptive statistics, Wilcoxon signed rank test were used in the data analysis by SPSS 18.0. Results: Muscle strength and balance were higher, and pain level were lower than before the line dance program. However, flexibility and mood state were not significantly changed after the line dance program. Conclusion: Findings of this study indicated that the 12-week line dance program were effective on muscle strength, balance, and pain relief in the post-menopausal women. Future study is needed to explore the effect of the line dance program on flexibility and mood state with the post-menopausal women.