In this study, the performance of mobile air conditioner(MAC) systems to which the refrigerants R134a and R1234yf were used was evaluated to compare the characteristic of automotive refrigeration cycles with refrigerant. The experimental setup of a MAC consists of an belt driven compressor, a condenser, an evaporator and a block type thermal expansion valve. The drop-in test on MAC were carried out under variable compressor speed from 800 to 2500 rpm. Performance test by using R1234yf and R134a in the same system revealed low the charge amount and mass flow rates for using R1234yf, that is, up to 10% and 17%, respectively. The compressor discharge temperature of R1234yf is $8^{circ}C$ lower than that of R134a. The cooling capacity with R1234yf system decreased by 4~7% compared with R134a system. In addition, The COP of R1234yf system is lower 3~4% than that of R134a system.