The purpose of this research is to investigate the heat transfer enhancement by the multiple tripping wires installed on a flat plate. Naphthalene sublimation technique is used to measure the local mass transfer coefficients, and then heat transfer coefficients are calculated using heat/mass transfer analogy. Experiments are performed for the developed and developing flow conditions. Local and average heat transfer coefficients from the flat plate with three tripping wires are compared with those of no and one tripping wire. Remarkable heat transfer enhancements are found resulting from the boundary layer separation by the tripping wires, especially in the laminar boundary layer.