Purpose: This study examines the difference of optimism, self-esteem and depression to verify an effect of laughter therapy program for menopausal women. Methods: A quasi-experimental nonequivalent control group pre / post-test design was used. The participants (n=57) were 28 in the experimental group and 29 in the control group. The data were collected in December of 2011. The experimental group participated in laughter therapy for 30 min per session, five times a week for the period of 2 weeks. The questionnaires were used to measure pre / post-test optimism, self-esteem, and depression of menopausal women. Results: The results showed that laughter therapy was effective in increasing optimism (p= .020) and self-esteem (p<.001). And the depression of menopausal women decreased (p= .003). Conclusion: The result of the study indicates that laughter therapy for menopausal women may be an effective nursing intervention to improve optimism, self-esteem and depression of menopausal women.