To understand oxygenated fuel characteristics including spray penetration length and spray angle at a real engine ambient pressure condition, DME was injected into a high pressure chamber by a piezo injector common rail system. The piezo injector common rail system was able to apply steady injection pressure, rapid response, and accurate injection quantity. Injection and ambient pressure were varied to confirm a relation with spray form. Using a direct photographing technique, development process of DME spray was captured. DME injection quantity was enlarged linearly as increasing of the injection pressure. In the high pressure chamber, when the injection pressure was enlarged the penetration length and velocity were increased due to a big momentum of fuel particle at the same ambient pressure. When ambient pressure was increased, the DME spray penetration length and velocity were decreased since the high ambient density of nitrogen was acted as a resistance. Although the ambient pressure and injection pressure were varied, each case of spray angle was almost same since the spray angle had a connection of the injector nozzle geometry.