- HPLC-UV에 의한 진득찰과 털진득찰의 Kirenol 정량분석
- ㆍ 저자명
- 누그로호 아궁,이경태,박희준,Nugroho. Agung,Lee. Kyung-Tae,Park. Hee-Juhn
- ㆍ 간행물명
- 생약학회지
- ㆍ 권/호정보
- 2012년|43권 4호|pp.286-290 (5 pages)
- ㆍ 발행정보
- 한국생약학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
Many diterpenoids from Siegesbeckia species (Compositae) and their anti-inflammatory actions have been examined. In this research, high-performance liquid chromatography-ultraviolet spectrophotometer (HPLC-UV) method was used to compare the quantitative level of kirenol (ent-pimarane-type diterpenoid) in the aerial parts of Korean S. glabrescens and S. pubescens and the Chinese Siegesbeckiae Herba. Fingerprints of the two HPLC chromatograms of Korean S. glabrescens and S. pubescens were similar, but considerably different from Chinese Siegesbeckiae Herba. The content of kirenol in S. pubescens ($16.51{pm}0.10$ mg/ml dry weight as mean${pm}$RSD) was higher than S. glabrescens ($13.48{pm}0.12$ mg/g dry weight). These values were considerably higher than the Chinese Siegesbeckiae Herba ($1.55{pm}0.74$ mg/g dry weight). Thin layer chromatography (TLC) analysis demonstrated the containing of kirenol in the three plant materials, but the presence of siegeskaurolic acid (entkaurane-type diterpenoid) only in the Chinese Siegesbeckiae Herba.