We cloned and sequenced the cDNA encoding the $FSH{eta}$ and $LH{eta}$ subunits from the pituitary of the blacktip grouper Epinephelus fasciatus, which regulate vitellogenesis and maturation in vertebrates, to achieve stable and healthy gametes. The full-length cDNAs of $FSH{eta}$ and $LH{eta}$ were 571 bp and 617 bp, encoding 120 amino acid (aa) and 147 aa proteins, respectively. The deduced amino acid sequences of $FSH{eta}$ and $LH{eta}$ were highly homologous (68-97%) to those of other Perciformes; E. bruneus, Dicentrarchus labrax, Thunnus thynnus, and Pseudolabrus sieboldi. Phylogenetic analysis showed that the deduced $FSH{eta}$ and $LH{eta}$ amino acid sequences were categorized as a distinct subunit in the $GTH{eta}$ family, and are closely related to the teleostei $FSH{eta}$ and $LH{eta}$, respectively. $FSH{eta}$ and $LH{eta}$ mRNA exhibited high abundance in the pituitary gland and low in other brain areas, but were not present in peripheral tissues, as determined by RT-PCR.