The purpose of this study was to identify the negative factors affecting personnel u-Learning acceptance and to analyze the interrelation among the factors in this research model. The two independent variables avoidable convenience and reliant convenience, based on pilot test results, and learning performance and perceived interactivity, based on the relevant literature, are used to examine the research model. The research problem was tested with data collected from 577 respondents in 23 universities. This study developed and empirically analyzed a model representing the relationship by using the Structural Equation Model. The major findings of this study are, firstly, that the higher reliant convenience is negatively affecting the degree of system use and learner’s satisfaction, whereas avoidable convenience is only affecting the learner’s satisfaction. Secondly, the higher learning performance and stronger perceived interactivity affects the degree of system use as well as learner’s satisfaction. Finally, the degree of system use affects the learner’s satisfaction.