Objective: The purpose of this study is to evaluate the potential risk of shoulder muscle at particular working postures in sitting. Background: The cause of shoulder pain needs to be specifically studied in relation with particular shoulder postures to prevent shoulder MSDs in workplace. Method: In this study MVC, fatigue and subjective workload were investigated depending on the change of shoulder posture. An experiment was designed to evaluate the six shoulder muscles at nine shoulder postures including the combination of 30(adduction), 0, 30(abduction) degrees and 60, 90, and 120 degrees of shoulder flexion. Surface electrodes were attached to the middle trapezius, inferior middle trapezius, anterior deltoid, posterior deltoid, serratus anterior and teres major. Thirteen subjects participated in the experiment. Dependent variables were RPE (rating of perceived exertion), MVC(maximum voluntary contraction) and MPF(mean power frequency) shift by EMG (electromyography). Results: The middle trapezius and inferior middle trapezius were not significantly fatigued at all postures. The decline of MPF slope was less than 10% at all postures. The anterior deltoid was significantly fatigued all postures. The decline of MPF slope was more than 10% at all postures. The posterior deltoid was significantly fatigued 30 degrees of adduction and 90 degrees of flexion. And, neutral and 30 degrees of abduction postures were fatigued more than 90 degrees of flexion. The serratus anterior was significantly fatigued except for 30 degrees of adduction and 60 degrees of flexion posture. The teres major was significantly fatigued except for neutral and 60 degrees of flexion, 30 degrees of abduction and 60 degrees of flexion posture. Conclusion: It was found that a certain muscle was fatigued fast at particular posture compared to other muscles, which would mean that a certain shoulder muscle at particular posture could be easily exposed to the risk of musculoskeletal disorders than other muscles. Application: It is expected that the result can be applied to design workplace using shoulder muscles.