본 연구는 담수미세조류의 일종인 클로렐라를 적색 발광다이오드를 이용하여 효율적으로 반연속 배양하기 위한 조건을 찾기 위해 수행되었다. 클로렐라 배양에 가장 효율적인 적색 LED를 사용하여 반연속배양을 실시하였으며, 배양 중 인과 질소가 급격히 감소하였으며 이는 클로렐라의 성장과 반비례의 관계를 보여 주었다. 효율적인 반연속 배양을 위해 인과 질소의 농도를 증가시킨 배지를 사용하여 배지교체 주기를 연장시켜 배양 효율성을 높였다. 배지교체 시 클로렐라의 셀 농도는 지속적으로 증가하였으나, 셀 크기는 감소하였다. 셀 농도와 셀 크기를 고려할 때 배양 전과정을 통해 생산되는 바이오매스의 양은 일정하게 유지되었다.
This study was performed to determine optimum conditions of semi-continuous cultivation of chlorella sp. FC-21 cultivated under red light emitting diode (LED). Semi-continuous cultivation was conducted using red LED because red LED was found to be the best light source for chlorella sp. FC-21. During cultivation, phosphate and nitrogen were quickly diminished where cell concentration of chlorella was inversely proportional to the concentrations of phosphate and nitrogen in culture solution. To increase the period of dilution of culture solution, additional amounts of phosphate and nitrogen were inserted in the culture solution to increase the concentrations of phosphate and nitrogen. The cell concentrations of chlorella increased in the modified culture, but cell diameter was diminished as the dilution of culture was periodically conducted. When considered the cell concentration and cell diameter during the cultivation, amount of biomass produced was maintained constant.
