Kichulchoia brevifasciata belonging to family Cobitidae is an endemic Korean freshwater fish and restricted to very small areas such as Goheung peninsula, and Geogum Island and Geumo Island flowing into the South Sea. As a decrease of its population was further accelerated, a close investigation on the distribution was required. Among a total of 21 sites surveyed, only 5 sites were confirmed in which K. brevifasciata existed. Based on this result, we classified the 5 sites into 3 categories: Being worthy of preserved, being subject to threaten and staying in good condition.