The aims of the present study were to estimate the possibility for toxicity test and compare acute toxicity of potassium dichromate in the larva stage of Neocaridina denticulata, Daphnia magna and the juvenile stage of Oryzias latipes. N. denticulate, a freshwater shrimp lives in Korea, is an indigenous species and considered to be useful for toxicity test. D. magna and O. latipes were recommended as a test species for the OECD test guideline. The 96 h-$LC_{50}$ potassium dichromate value was 0.62 mg $L^{-1}$ for the larva stage of N. denticulata and 168.44 mg $L^{-1}$ for the juvenile stage of O. latipes. The 48 h-$EC_{50}$ value was 1.27 mg $L^{-1}$ for the D. magna. The study was confirmed higher sensitivity of the larva stage of N. denticulata to potassium dichromate compared to the D. magna and the juvenile stage of O. latipes.