본 연구는 느타리버섯 $850m{ell}$ 배양용기의 뚜껑종류 및 크기에 따른 배양중 이산화탄소농도변화, 수분함량, 유리당, 키친 함량 및 수량을 조사하였다. 배양중 이산화탄소 변화는 배양 뚜껑 종류와는 관계없이 배양 경과 8~9일경 이산화탄소발생량이 최고치에 도달하였고, 뚜껑종류별 이산화탄소누적 농도는 뚜껑 크기 29~41mm를 비교하였을 때 상하천천공은 6.5~4.0%, 하부천공은 9.0~6.5%로 하부천공이 이산화탄소농가 높게 측정되었다. 수분변화는 구멍크기가 클수록 배지 내 수분 보유량은 감소하였다. 유리당과 키친의 변화는 상하천공보다 하부천공에서 유리당 및 키친량이 많았고, 수량은 상하천공 23~33mm, 하천공 29mm에서 각각 15.8~21.2%, 20%가 증수되었다.
The plastic culture bottle cap types and accumulated concentration of carbon dioxide, media humidity in the process of medium culture, chitin content and yield were observed in Pleurotus ostreatus 850ml bottle in Iksan, Jeollabuk-do, Korea, during 2011. The concentration of carbon dioxide in the process of medium culture was the highest after 8~9 days cultivation irrespective of cap sizes and types. The accumulated concentration of carbon dioxide in size cap of 29~41mm was 6.5~4.0% in the upper-under perforation hole of cap and 9.0~6.5% in the under perforation hole of cap. The upper-under 23~33mm perforation hole and under 29mm perforation hole of caps in the 850ml bottle were best condition for cultivation of mushroom and increased fruit body, 15.8~21.2% and 20%, respectively. However, the upper-under & under 41mm perforation hole of fruit body were decreased 60.7% and 23.6%, respectively. Also it was weak, lose vitality and the lower of biologically activity substance because the upper medium humidity was too dry.
