본 연구는 버들송이버섯의 균사 생육 최적조건을 구명하여 액체종균을 제조하고 또한 톱밥종균의 영양원을 선발하기위하여 시험을 수행하였고, 선발된 종균에 대한 종균 접종량을 설정하여 병재배에 적합한 조건을 구명하고자 하였다. 버들송이버섯의 톱밥종균은 미송톱밥(70%)+밀기울(30%)의 비율, 포플러톱밥(80%)+옥수수가루(20%) 조건으로 배합할 때 균사 생육이 양호하였다. 액체종균에 대한 영양원 선발 및 첨가량은 백설탕 1.0~1.5%, yeast extract $0.7g/{ell}$, 콩가루 $0.17g/{ell}$, 무기물은 $MgSO_4{cdot}7H_2O;0.3g/{ell}$, $KH_2PO_4;0.5g/{ell}$, $K_2HPO;1.2g/{ell}$가 최적 조건이었다. 이때 선발된 배지의 접종량은 액체 및 톱밥종균을 $25m{ell}/850m{ell}$과 $20{sim}25g/850m{ell}$로 접종 할 때 자실체 발생이 양호하였다.
Studies were made to optimize the media composition and cultural condition for mycelial growth of Agrocybe cylindracea. Sawdust spawn of media composition for optimal growth was found to be pine sawdust combination of 30% wheat bran and poplar sawdust combination of 20% corn bran were the best of the optimal combination. The optimal concentration of white sugar was 1.0~1.5%. The nitrogen sources was found to be yeast extract and soybean powder. Also, optimal concentration were $0.7g/{ell}$ and $0.1g/{ell}$, respectively. The mineral sources of optimal medium compositions were $MgSO_4{cdot}7H_2O;0.3g/{ell}$, $KH_2PO_4;0.5g/{ell}$ and $K_2HPO;1.2g/{ell}$. Optimal amount of inoculum for cultivation of A. cylindracea were $20{sim}25g/850m{ell}$ and $25m{ell}/850m{ell}$ in the sawdust spawn and liquid spawn, respectively.
