To increase the extraction yield and biological effect of antler by fermentation, probiotics fermenting antler were screened from rice shells. Of screened Bacillus species, the most potently antler-degrading probiotics was Bacillus KH-07. The supernatant yield of fermented antler is highest. Bacillus KH-07 may belong to Bacillus licheniformis by biochemical and 16S rDNA sequencing analyses. The KH-07-fermented antler (50 mg/kg) increased the anti-fatigue effect 2.3-fold compared to that of non-treated antler. Furthermore, the KH-07-fermented antler improved $CCl_4$-induced liver injury in mice. Based on these findings, the extraction yield and biological effect of antler can be increased by KH-07 fermentation.