Human peroxisome proliferator-activated receptor gamma ($hPPAR{gamma}$) has been implicated in numerous pathologies, including obesity, diabetes, and cancer. In this study, we verified that amentoflavone is an agonist of $hPPAR{gamma}$ and probed the molecular basis of its action. It was demonstrated that amentoflavone bound $hPPAR{gamma}$ with high (picomolar) affinity and increased the binding between $hPPAR{gamma}$ and steroid receptor coactivator-1 (SRC-1) by approximately 4-fold. Based on a docking study, for the first time, we propose a model of amentoflavone and $hPPAR{gamma}$ binding in which amentoflavone forms three hydrogen bonds with the side chains of His323, Tyr327, and Arg280 in $hPPAR{gamma}$ and participates in two hydrophobic interactions.