Many previous studies reported acoustic parameters of English vowels produced by a clear speaking style. In everyday usage, we actually produce speech sounds with various speaking styles. Different styles may yield different acoustic measurements. This study attempts to examine pitch and formant trajectories of eleven English vowels produced by nine American males in order to understand acoustic variations depending on clear and conversational speaking styles. The author used Praat to obtain trajectories systematically at seven equidistant time points over the vowel segment while checking measurement validity. Results showed that pitch trajectories indicated distinct patterns depending on four speaking styles. Generally, higher pitch values were observed in the higher vowels and the pitch was higher in the clear speaking styles than that in the conversational styles. The same trend was observed in the three formant trajectories of front vowels and the first formant trajectories of back vowels. The second and third trajectories of back vowels revealed an opposite or inconsistent trend, which might be attributable to the coarticulation of the following consonant or lip rounding gestures. The author made a tentative conclusion that people tend to produce vowels to enhance pitch and formant differences to transmit their information clearly. Further perceptual studies on synthesized vowels with varying pitch and formant values are desirable to address the conclusion.