Objectives : This study was purposed to estimate prevalence of upper extremity musculoskeletal symptoms, level of occupational stress and their relationship among dental hygienists. Methods : This questionnaire study was performed during May to October 2010 with 317 dental hygienists worked in Busan and Gyeongsangnam-do recruited as study population. The occupational stress and upper extremity musculoskeletal symptoms were accessed using questionnaire of the Korean Occupational Stress Scale and musculoskeletal symptoms from the Korea Occupational Safety Health Agency. Data analysis was performed with the descriptive analysis, chi-square test, and multiple logistic regression analysis using SPSS(ver 19.0K)program. Results : The prevalence of upper extremity musculoskeletal symptoms and the level of occupational stress were 79.2% and $52.67{pm}4.86$ in dental hygienists. By multiple logistic regression analysis, job insecurity(OR=2.86, 95% CI: 1.38-5.94 in stress high risk group; reference-stress low risk group) and organizational system(OR=3.32, 95% CI: 1.55-7.08 in stress high risk group; reference-stress low risk group) were associated with upper extremity musculoskeletal symptoms in dental hygienists. Conclusions : The overall prevalence of upper extremity musculoskeletal symptoms and level of occupational stress in dental hygienists were relatively high and some sub-scales of occupational stress were related with upper extremity musculoskeletal symptoms. Not only previously reported risk factors of musculoskeletal symptoms but also occupational stress should consider to prevent and improve upper extremity musculoskeletal symptoms in dental hygienists.