The objective of this study was to investigate the oxygen transfer characteristics of an ejector aeration system. In order to evaluate the oxygen transfer performance of the ejector aeration system, a comparative experiment was conducted on a conventional blower aeration system. The effect of entrained air flow rate and aerating water temperature on the oxygen transfer efficiency was investigated. The dissolved oxygen concentration increased with increasing entrained air flow rate, but decreased with increasing aerating water temperature for two aeration systems. The volumetric mass transfer coefficient increased with increasing entrained air flow rate and with increasing aerating water temperature for both aeration systems. The average mass transfer coefficient for the ejector aeration system was about 20% and 42% higher than that of the blower aeration system within the experimental range of entrained air flow rates and aerating water temperatures.