$Vibrio$ $vulnificus$, a gram-negative halophilic marine bacterium and opportunistic human pathogen, must withstand various environmental changes, especially simultaneous changes in temperature and salinity, from $25^{circ}C$/2.5% to $37^{circ}C$/0.9% (SCTS) upon entering the human body. In our previous study, SCTS stimulated $vvpE$ expression even in the background of a mutation in $luxS$ encoding LuxS enzyme for the biosynthesis of quorum-sensing (QS) signal molecule autoinducer-2 (AI-2), suggesting that the A1-2-mediated QS system is partially involved in the SCTS-mediated change of $vvpE$ expression. In this study, we examined the effects of the QS master regulator SmcR on SCTS-mediated changes in $vvpE$ expression and extracellular VvpE production. SCTS stimulated $V.$ $vulnificus$ growth, but with no increase in maximal growth levels. The SCTS-mediated prolongation of the stationary growth phase resulted in a significant increase in growth phase-dependent $smcR$ and $vvpE$ expressions. A mutation in $smcR$ seriously repressed $vvpE$ expression, but had no significant effect on $V.$ $vulnificus$ growth. However, the $smcR$ mutation only partially attenuates SCTS-mediated changes in $vvpE$ expression. These results indicate that SCTS stimulates the expressions of $smcR$ and $vvpE$ by stimulating $V.$ $vulnificus$ growth, and that SmcR is only partially involved in SCTS-mediated changes in $vvpE$ expression.