An experimental investigation was presented to determine the optimum configuration of influential parameters (concentrate volume, flow rate, temperature, initial lactate concentration, voltage, and impurities) for the best performance. AMX-CMX ion-exchange membranes were used in all experiments. Temperature was found to possess an important role in the increase of lactate recovery, and it was not increased to high values due to membrane destruction. When the higher voltage was applied to electrodialyzer, the better performance of electrodialysis was observed due to enhancement of the driving force. It was found that, to achieve an optimum operating condition, feed volume, concentrate volume, flow rate, temperature, initial lactate concentration in concentrate, and voltage should be 2 L, 1 L, 0.8 L/min, $32^{circ}C$, 1 g/L, and 15.0 V, respectively. Under these optimized conditions, 97% of lactate was successfully recovered from the fermentation broth, where the lactate flux and energy cost were $7.2{pm}0.6$ moles/$m^2$/h and 0.25 kWh/kg, respectively. The results of experiments indicate that the lactic acid in the fermentation broth can be economically purified by electrodialysis.