Thermal protection of firefighter protective clothing is greatly influenced by the air gap entrapped and moisture in clothing. In this paper, the effects of air gap size and position on thermal protective performance exposed to $84kW/m^2$ heat source were investigated. Water was also added to thermal liner to understand the effect of air gap coupled with moisture on thermal protection. It was indicated that the TPP of fabrics system increased with the air gap size. The air gap position also greatly influenced the heat transfer during exposure to flash fire. Moisture added weakened the positive effect of air gap size when the air gap exists far from heat source, and almost eliminated the favorable effect of air gap position. However, when there is no air gap or small air gap between outer shell fabric and moisture barrier, moisture increased the thermal protection performance of multilayer fabrics system. The results obtained suggested that certain air gap entrapped in fabrics system and clothing microclimate could improve thermal protection, and the complicated effect of moisture should also be considered.