Purpose: This study was done to examine the importance of health promotion behavior for infants and toddlers as perceived by mothers of children in early childhood, and graduate students in nursing and to provide information for health promotion in early childhood. Methods: This study was a cross-sectional survey research study. The participants were 91 mothers of children in early childhood, and 115 graduate students in nursing. Results: The comparisons showed that graduate students in nursing reported higher perception of the importance health promotion behavior for infants and toddlers than did the mothers. In health promotion behavior, the highest score was in the category of safety. Conclusion: Providing information by nurses is necessary to promote health promotion behavior for mothers of children in early childhood. Therefore, it is important for healthcare professionals to develop effective programs for these mothers who want to promote good health promotion behavior in their children.