In this study, fatigue life of the motor block bracket units for KTX-Sancheon trains was assessed. Design evaluation for railway structures was performed based on the UIC 566 regulation, and test and evaluation of fatigue life in welded parts was performed in accordance with standard ERRI B 12/RP17 and ERRI B 12/RP60. The actual vehicle dynamic stress testing was executed in KTX-Sancheon service line with the service operating speed. The dynamic stress was measured with commercial data acquisition system (MGC plus). The cumulative damage was evaluated by applying standard BS 7608 - Class F and cycle counting was used rain-flow counting method. As a result, the motor block bracket units for KTX-Sancheon trains was designed to fit the regulation and the safety of fatigue life for 30 years, assuming that KTX-Sancheon trains travels 600,000km annually, were confirmed under current operating conditions.